Week #

Date

Turn in  lab #(s)

Investigation #

Laboratory Topics

1

1/19

1 and Pipetting Handout

Introduction and Orientation to the genetics lab

Drosophila and Maize Experiments in Genetics: Monohybrid and Dihybrid crosses

Class code for Section 1 : 4465347

Class code for Section 2:  4466143

Drosophila Development site : http://www.sdbonline.org/fly/aimain/1aahome.htm

Micropipette Video : http://www.sigmaaldrich.com/life-science/cell-culture/learning-center/cell-culture-videos/the-micropipette.html

2

1/26

2 & 4

Principles of Probability Finish the probability lab

Cell Reproduction: Mitosis

3

2/2

1

Handout and start 26

Genetic Material: Isolation of DNA & NCBI and Genomic Data Mining

extract DNA from anything living University of Utah 

4

2/9

2 and 4

15 & continue Lab 26

Restriction Endonuclease Digestion, Gel Electrophoresis, and analysis used to Map Restriction Fragments

NCBI and Genomic Data Mining

Lab 2: Restriction enzyme cleavage and electrophoresis

http://www.phschool.com/science/biology_place/labbench/lab6/concepts2.html

Information sheet for the 1kb dna ladder from New England Biolabs

Photograph of Restricted Lambda Spring 2008

How to graph on log paper : http://www.physics.uoguelph.ca/tutorials/GLP/

5

2/16

DNA Lab

17

Transformation of E.coli

6

2/23

15 & 26

7 & 8

Polytene Chromosomes from Drosophila Salivary Glands

Sex Chromosomes and Gene Transmission

Polytene Chromosomes

done with Drosophila melanogaster

Drosophila polytene chromosome micrograph

Sex linked inheritance from answer.com scroll down and read the articles http://www.answers.com/topic/sex-linked-inheritance

Hemophilia

7

3/2

17

3 & 5

Chi Square Test

Meiosis in Animals: Oogenesis and Spermatogenesis

http://www.biosci.ohiou.edu/introbioslab/Bios170/170_6/170_6.htm scroll down to see meiosis in Ascaris photos

http://bio-ditrl.sunsite.ualberta.ca/search/results/?p_field=keywords&p_find=Orthoptera&Page=3 insect testes

http://highered.mcgraw-hill.com/sites/0072437316/student_view0/chapter12/animations.html

http://it.stlawu.edu/~mtem/devbiol/DBLAB1.HTM

http://www.umanitoba.ca/faculties/science/biological_sciences/lab5/biolab5_4.html#

http://instruct1.cit.cornell.edu/courses/biog105/images/106slides/unit1slides

8

3/9

7 & 8

9 &10

The Sex Check: A study of Sex Chromatin in Human Cells and Human Chromosomes

http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/S/SexChromosomes.html

Karyotyping Activity

Making a Karyotype

9

3/23

3 & 5

12&19

Genetics of Ascospores Color in Sordoria an Investigation of Linkage and Crossing Over using Tetrad Anaylsis

Chromatographic Characterization of Drosophila melangogaster Mutants

• http://botit.botany.wisc.edu/courses/botany_130-fall2004/Lab-manual/h-Meiosis.pdf
• http://botit.botany.wisc.edu/courses/botany_130/Lab_topics/bk-meiosis.pdf

Drosophila Mutants:

• Fly eye chromotograph photographs fall 2009 Tuesday
  • photo 1
  • photo 2
  • photo 3
  • photo 4
• Fly eye chromotograph photographs fall 2009 Wednesday
  • photo 1
  • photo 2
• fly eye chromatograph photographs spring 2008 the best images are DSC2968, and IMG 0306, 0309, 0311 and 0316
• fly eye chromatograph photographs fall 2007
• http://www.accessexcellence.org/AE/AEPC/WWC/1994/characterizing_drosophila.html
• http://biotech.biology.arizona.edu/labs/Drosophila_teach.html

March 15-19, 2010 is SPRING Break - April 1, 2010 is the last day to drop classes

10

3/30

9 &10

PCR Handout

Human VNTR Polymorphism AT – 2 grades for this lab

http://www.dnalc.org/ddnalc/resources/shockwave/pcranwhole.html

http://biology.kenyon.edu/courses/biol09/PCR/Dna/FRAMES/PCR2.htm

http://www.cybertory.org/exercises/forensics/paternity.html

11

4/6

12&19

PCR gel analysis

&  25

TA will run samples on a gel, students will evaluate their samples and answer the questions

Start work on Applied Human Genetics Part I. B. 1 make a pedigree as described

12

4/13

Handout - remind us to give you a copy! 

Plaques

Working with Bacteriophages

t4 plate with ruler to measure size of plaques

t4r plate with ruler to measure size of plaques

both t4 and t4r plates to count
 

13

4/20

PCR

22 & 23

Polygenic inheritance: Fingerprint Ridge Count Population Genetics: The Hardy Weinberg Principle

http://staff.jccc.net/pdecell/evolution/polygen.html

Population Genetics made Simple http://www.cs.unc.edu/~plaisted/ce/genetics.html

http://www.ndsu.nodak.edu/instruct/mcclean/plsc431/popgen/popgen1.htm

Population Biology Simulations http://darwin.eeb.uconn.edu/simulations/simulations.html

14

4/27

Plaques

24 and 25

Part II only – Genetic Drift and Fixation of an allele

Applied Human Genetic

15

5/4

22 & 23

24 & 25

Final

EXAM

Turn in the last lab reports and take finals